On statistical tests of functional connectome fingerprinting

Background: Surgical removal of malignancies may enhance the risk recurrence but mechanisms remain poorly understood. Neutrophils, first line responders after surgical stress, can facilitate cancer progression. Neutrophils expel nuclear DNA studded with proteins to form web-like structures known as Neutrophil Extracellular Traps (NETs) in response stress. Here we aim characterize role NETs promoting metastatic growth stress that is currently entirely unknown. Methods: We used a standardized mouse model partial hepatic ischemia reperfusion, stimulate directly or five days splenic injection MC38 colon carcinoma cells. Results: found NET formation significantly increased immediate post-operative period humans undergoing curative liver resection for colorectal metastases. The metastases was 4.22 times higher patients formation. In murine model, demonstrated deposition and this associated both already established micrometastatic disease. vitro, increase growth, invasion, migration adhesion multiple cell lines. All above effects were abrogated by inhibition DNAse. exert their pro-tumorigenic releasing HMGB1 activating TLR-9 dependent pathways addition, tumors continue grow, hypoxia within tumor microenvironment induces further neutrophil recruitment accelerates growth. Conclusions: These findings implicate process metastasis progression surgery identifies potential therapeutic targets. Citation Format: Samer Tohme, Hai Huang, Alexis Chidi, Richard Simmons, Allan Tsung. extracellular traps following [abstract]. In: Proceedings AACR Special Conference on Tumor Metastasis; 2015 Nov 30-Dec 3; Austin, TX. Philadelphia (PA): AACR; Cancer Res 2016;76(7 Suppl):Abstract nr A30.